Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2e. Descriptive Summary for Subjects Aged 50-66 Years



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

32

10.9

3.9

0.7

10.7

7.8, 13.3

4.0, 24.8


IgD+ Memory B cells

32

8.4

4.4

0.8

7.5

4.5, 11.7

1.7, 17.8


IgD- Memory B cells

32

19.3

9.2

1.6

16.2

13.2, 24.2

4.2, 45.4


IgD-CD27- B cells

32

7.8

4.9

0.9

6.6

4.2, 10.4

2.2, 22.8


Naïve B cells

32

64.5

12.5

2.2

66.5

60.9, 70.7

26.5, 85.7


Plasma cells

32

0.7

0.5

0.1

0.6

0.4, 0.9

0.1, 2.4


Plasmablasts

32

0.3

0.2

0.0

0.2

0.2, 0.4

0.1, 1.1


Transitional B cells

32

4.3

2.7

0.5

3.8

2.1, 5.9

0.8, 11.9

DCMNK

CD11c+CD123+ DC (DC)

32

7.5

10.4

1.8

3.1

1.0, 12.1

0.3, 52.4


CD11c+CD123+ DC (Live Scatter)

32

0.1

0.2

0.0

0.0

0.0, 0.1

0.0, 0.8


CD14+ Monocytes

32

80.5

7.6

1.3

81.2

75.5, 86.3

63.6, 93.1


CD16+CD14- Monocytes

32

9.2

4.6

0.8

8.4

6.5, 12.7

1.9, 21.7


CD16+CD56+ NK cells

32

78.9

10.7

1.9

81.2

72.7, 88.1

45.0, 91.1


CD16+CD56- NK cells

32

10.0

7.2

1.3

8.2

5.4, 14.2

1.8, 30.9


CD16-CD56+ NK cells

32

10.2

4.9

0.9

8.7

6.0, 12.4

4.7, 22.5


Classical Monocytes

32

77.9

8.1

1.4

78.3

73.4, 83.0

60.4, 92.3


Dendritic cells (Lin-)

32

1.7

0.8

0.1

1.5

1.2, 2.1

0.6, 4.4


Dendritic cells (Live Scatter)

32

1.2

0.5

0.1

1.0

0.8, 1.4

0.4, 2.6


Monocytes

32

11.5

4.5

0.8

11.5

7.5, 14.5

3.3, 23.2


Myeloid DC (DC)

32

44.5

12.5

2.2

44.2

35.8, 53.0

19.7, 68.3


Myeloid DC (Live Scatter)

32

0.5

0.2

0.0

0.5

0.4, 0.6

0.1, 1.4


NK cells

32

9.0

4.9

0.9

8.7

4.7, 11.7

2.2, 19.4


Plasmacytoid DC (DC)

32

25.6

11.0

2.0

23.3

17.4, 32.0

3.9, 51.4


Plasmacytoid DC (Live Scatter)

32

0.3

0.2

0.0

0.2

0.2, 0.4

0.1, 1.0

Tcell

Activated CD4+ T cells

30

1.7

0.9

0.2

1.5

1.2, 1.8

0.8, 4.6


Activated CD8+ T cells

30

5.7

3.6

0.7

4.9

3.0, 6.7

0.7, 15.8


Activated Central Memory CD4+ T cells

30

1.5

0.8

0.1

1.2

1.0, 2.0

0.6, 3.7


Activated Central Memory CD8+ T cells

30

4.2

2.5

0.4

3.5

2.7, 4.7

1.2, 11.2


Activated Effector CD4+ T cells

30

3.5

2.7

0.5

2.6

1.5, 5.5

0.4, 9.9


Activated Effector CD8+ T cells

30

8.2

4.9

0.9

6.2

5.1, 11.0

2.5, 21.9


Activated Effector Memory CD4+ T cells

30

6.0

3.3

0.6

5.1

3.3, 8.3

1.6, 14.0


Activated Effector Memory CD8+ T cells

30

11.1

6.4

1.2

9.5

7.6, 12.8

1.4, 33.7


Activated Naive CD4+ T cells

30

0.9

1.1

0.2

0.5

0.3, 1.3

0.1, 5.1


Activated Naive CD8+ T cells

30

2.3

1.8

0.3

1.9

0.8, 3.6

0.3, 7.9


Average CD4+ T cells

32

56.9

7.6

1.4

56.8

53.5, 60.5

35.8, 72.8


Average Lymphocytes

32

59.4

11.4

2.0

62.9

49.9, 69.5

34.5, 77.5


Average T cells

32

79.6

5.1

0.9

79.2

75.9, 84.2

70.3, 88.6


CD8+ T cells

30

20.0

7.8

1.4

20.6

13.6, 23.6

5.9, 37.9


Central Memory CD4+ T cells

30

48.5

11.7

2.1

48.3

39.4, 55.5

26.7, 77.6


Central Memory CD8+ T cells

30

13.1

6.5

1.2

12.1

8.0, 16.0

3.9, 32.8


Effector CD4+ T cells

30

4.5

5.6

1.0

1.7

0.7, 6.9

0.0, 25.5


Effector CD8+ T cells

30

37.6

16.6

3.0

36.3

27.0, 48.8

1.7, 73.0


Effector Memory CD4+ T cells

30

11.3

5.8

1.1

11.0

6.6, 15.4

2.3, 25.4


Effector Memory CD8+ T cells

30

10.8

6.6

1.2

9.1

6.5, 15.6

2.5, 33.6


Naive CD4+ T cells

30

35.3

14.6

2.7

34.7

23.4, 45.9

6.0, 60.3


Naive CD8+ T cells

30

38.5

16.4

3.0

33.7

28.6, 46.2

16.4, 81.7

Tfh

Activated T follicular helper

32

0.4

0.2

0.0

0.4

0.3, 0.6

0.1, 0.9


Medium Activated T follicular helper

32

16.5

4.5

0.8

16.6

13.2, 19.8

8.1, 25.5


T follicular helper

32

13.0

4.6

0.8

11.9

9.2, 16.9

6.4, 24.3


T follicular helper type 1

32

24.1

6.6

1.2

23.9

21.0, 27.9

7.4, 37.7


T follicular helper type 17

32

36.1

7.6

1.4

36.2

30.8, 39.7

22.3, 59.1


T follicular helper type 2

32

23.7

5.6

1.0

23.0

20.3, 27.6

10.6, 33.0


T follicular regulatory

32

4.4

2.3

0.4

3.8

3.1, 4.8

2.0, 13.6

Treg

Activated Memory Treg cells

32

47.4

9.8

1.7

48.1

40.2, 54.0

29.0, 70.1


Activated Naïve Treg cells

32

2.0

3.2

0.6

1.3

0.5, 2.2

0.1, 17.0


Effector Treg cells

32

74.6

11.5

2.0

74.9

67.5, 83.4

42.8, 91.6


HLA-DR- Memory Treg cells

32

42.5

10.1

1.8

43.6

35.4, 48.4

21.1, 62.3


HLA-DR- Naive Treg cells

32

8.1

5.4

1.0

7.6

4.6, 10.1

2.1, 32.8


Treg cells

32

7.1

2.1

0.4

7.0

5.4, 9.0

3.2, 10.5

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile