Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2d. Descriptive Summary for Subjects Aged 40-49 Years



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

30

10.5

4.3

0.8

10.0

6.9, 13.5

1.8, 23.7


IgD+ Memory B cells

30

6.5

4.2

0.8

5.1

3.9, 8.3

1.5, 17.5


IgD- Memory B cells

30

20.4

7.3

1.3

20.8

14.4, 24.7

7.0, 36.6


IgD-CD27- B cells

30

9.2

3.8

0.7

8.8

6.6, 12.0

3.6, 15.8


Naïve B cells

30

63.9

10.7

1.9

65.0

55.2, 69.7

38.7, 86.6


Plasma cells

30

1.1

0.8

0.1

0.7

0.5, 1.8

0.3, 2.9


Plasmablasts

30

0.4

0.3

0.1

0.3

0.2, 0.7

0.1, 1.3


Transitional B cells

30

4.8

4.8

0.9

3.2

1.7, 5.8

0.7, 21.1

DCMNK

CD11c+CD123+ DC (DC)

30

9.5

10.0

1.8

4.9

0.9, 16.8

0.2, 30.2


CD11c+CD123+ DC (Live Scatter)

30

0.1

0.2

0.0

0.1

0.0, 0.3

0.0, 0.5


CD14+ Monocytes

30

81.3

7.2

1.3

82.3

79.6, 85.8

54.3, 90.4


CD16+CD14- Monocytes

30

9.0

5.7

1.0

7.1

4.4, 13.1

0.5, 22.5


CD16+CD56+ NK cells

30

73.9

13.3

2.4

77.0

67.6, 84.2

46.8, 91.1


CD16+CD56- NK cells

30

12.3

9.4

1.7

9.0

5.9, 14.9

3.6, 39.5


CD16-CD56+ NK cells

30

12.7

9.0

1.7

10.5

6.0, 15.3

3.7, 43.3


Classical Monocytes

30

78.5

8.1

1.5

80.3

76.6, 84.1

51.3, 88.0


Dendritic cells (Lin-)

30

2.0

0.8

0.1

2.0

1.3, 2.5

0.9, 3.9


Dendritic cells (Live Scatter)

30

1.4

0.5

0.1

1.5

0.9, 1.8

0.7, 2.5


Monocytes

30

11.2

3.8

0.7

11.2

8.2, 14.2

4.9, 19.9


Myeloid DC (DC)

30

47.2

13.1

2.4

46.1

38.0, 56.4

18.8, 75.7


Myeloid DC (Live Scatter)

30

0.7

0.4

0.1

0.7

0.3, 0.9

0.2, 1.7


NK cells

30

9.1

4.5

0.8

8.0

5.6, 10.9

2.5, 21.9


Plasmacytoid DC (DC)

30

23.2

11.9

2.2

19.7

13.7, 33.4

9.0, 55.0


Plasmacytoid DC (Live Scatter)

30

0.3

0.2

0.0

0.3

0.2, 0.3

0.1, 0.8

Tcell

Activated CD4+ T cells

28

1.8

1.0

0.2

1.7

1.2, 2.1

0.6, 5.8


Activated CD8+ T cells

28

4.6

3.1

0.6

3.6

2.5, 4.4

1.9, 13.3


Activated Central Memory CD4+ T cells

28

1.6

0.7

0.1

1.4

0.9, 1.8

0.7, 3.7


Activated Central Memory CD8+ T cells

27

4.7

2.3

0.4

4.1

3.0, 6.5

1.6, 9.4


Activated Effector CD4+ T cells

28

4.0

3.1

0.6

3.4

1.8, 5.5

0.5, 14.8


Activated Effector CD8+ T cells

27

6.7

4.9

1.0

4.6

3.5, 11.8

1.8, 18.7


Activated Effector Memory CD4+ T cells

28

5.9

3.6

0.7

5.1

4.2, 6.7

2.1, 21.8


Activated Effector Memory CD8+ T cells

27

11.5

7.3

1.4

9.4

6.5, 14.2

3.4, 33.7


Activated Naive CD4+ T cells

28

0.8

0.6

0.1

0.8

0.4, 1.2

0.1, 3.1


Activated Naive CD8+ T cells

27

1.7

1.3

0.3

1.5

1.1, 2.0

0.3, 5.6


Average CD4+ T cells

30

53.2

8.9

1.6

53.2

45.8, 59.1

37.5, 71.8


Average Lymphocytes

30

62.0

8.5

1.6

63.0

60.8, 67.4

37.1, 76.7


Average T cells

30

77.7

7.1

1.3

77.5

72.3, 82.7

64.6, 93.0


CD8+ T cells

28

19.9

5.2

1.0

20.7

15.9, 23.0

11.2, 31.2


Central Memory CD4+ T cells

28

49.5

9.7

1.8

50.1

45.0, 55.7

22.3, 67.5


Central Memory CD8+ T cells

27

14.2

6.7

1.3

12.8

9.2, 16.4

3.5, 30.9


Effector CD4+ T cells

28

3.1

4.0

0.7

1.7

0.6, 3.5

0.2, 17.3


Effector CD8+ T cells

27

28.7

14.5

2.8

24.8

16.9, 41.7

10.3, 61.3


Effector Memory CD4+ T cells

28

11.6

5.4

1.0

11.1

8.1, 14.7

2.9, 28.4


Effector Memory CD8+ T cells

27

13.4

5.7

1.1

13.9

9.3, 16.7

2.6, 24.2


Naive CD4+ T cells

28

35.5

11.4

2.2

33.5

26.9, 42.1

17.6, 59.7


Naive CD8+ T cells

27

43.7

16.8

3.2

47.0

28.7, 57.1

17.4, 78.6

Tfh

Activated T follicular helper

30

0.4

0.2

0.0

0.3

0.2, 0.5

0.1, 1.1


Medium Activated T follicular helper

30

16.4

3.1

0.6

16.6

14.2, 18.0

8.8, 22.0


T follicular helper

30

13.8

3.4

0.6

13.7

12.1, 15.7

7.9, 19.9


T follicular helper type 1

30

24.7

5.4

1.0

25.5

20.7, 28.4

12.9, 36.3


T follicular helper type 17

30

35.5

7.1

1.3

35.0

31.0, 38.7

21.2, 55.3


T follicular helper type 2

30

22.9

5.3

1.0

21.8

18.6, 27.2

14.0, 32.5


T follicular regulatory

30

5.3

1.8

0.3

5.3

4.2, 6.2

2.8, 10.1

Treg

Activated Memory Treg cells

30

46.2

10.1

1.8

44.2

39.7, 50.9

24.7, 67.7


Activated Naïve Treg cells

30

2.2

2.6

0.5

1.0

0.5, 2.6

0.1, 9.0


Effector Treg cells

30

68.4

11.3

2.1

68.8

64.0, 74.9

45.0, 92.1


HLA-DR- Memory Treg cells

30

43.6

10.2

1.9

41.0

36.5, 51.0

25.0, 67.0


HLA-DR- Naive Treg cells

30

8.0

5.1

0.9

6.6

4.9, 8.5

1.2, 21.5


Treg cells

30

7.8

1.8

0.3

7.8

6.5, 8.5

5.2, 14.4

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile