Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2b. Descriptive Summary for Subjects Aged 18-29 Years



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

32

10.9

4.5

0.8

9.9

8.0, 13.4

5.1, 22.9


IgD+ Memory B cells

32

7.3

4.1

0.7

6.4

4.5, 9.3

2.0, 18.3


IgD- Memory B cells

32

23.3

10.2

1.8

23.1

15.3, 27.5

8.3, 50.9


IgD-CD27- B cells

32

13.7

7.0

1.2

12.4

7.8, 18.6

3.7, 28.9


Naïve B cells

32

55.7

14.2

2.5

58.1

45.3, 67.1

20.9, 75.9


Plasma cells

32

1.6

0.8

0.1

1.5

1.0, 2.3

0.3, 3.7


Plasmablasts

32

0.6

0.3

0.1

0.5

0.3, 0.9

0.0, 1.3


Transitional B cells

32

3.8

2.8

0.5

3.3

1.4, 4.9

0.4, 10.5

DCMNK

CD11c+CD123+ DC (DC)

32

3.9

7.2

1.3

1.9

1.1, 3.2

0.2, 40.4


CD11c+CD123+ DC (Live Scatter)

32

0.1

0.1

0.0

0.0

0.0, 0.1

0.0, 0.7


CD14+ Monocytes

32

79.4

7.8

1.4

78.9

75.4, 85.9

53.7, 88.8


CD16+CD14- Monocytes

32

8.0

6.2

1.1

6.2

4.3, 11.8

0.5, 30.2


CD16+CD56+ NK cells

32

75.8

12.2

2.2

77.3

69.4, 84.8

25.4, 87.8


CD16+CD56- NK cells

32

7.9

6.4

1.1

6.0

3.8, 8.5

2.3, 31.9


CD16-CD56+ NK cells

32

15.5

11.3

2.0

12.1

8.7, 18.0

4.5, 65.7


Classical Monocytes

32

77.5

8.2

1.5

77.4

73.9, 84.6

51.7, 87.5


Dendritic cells (Lin-)

32

1.8

0.8

0.1

1.7

1.4, 2.3

0.4, 4.2


Dendritic cells (Live Scatter)

32

1.4

0.5

0.1

1.4

1.1, 1.8

0.3, 2.5


Monocytes

32

9.4

4.3

0.8

9.1

5.8, 11.6

2.4, 19.1


Myeloid DC (DC)

32

45.1

11.1

2.0

44.4

36.3, 51.7

22.6, 70.5


Myeloid DC (Live Scatter)

32

0.6

0.3

0.1

0.6

0.4, 0.8

0.1, 1.8


NK cells

32

7.8

4.4

0.8

6.4

4.8, 10.6

2.9, 19.8


Plasmacytoid DC (DC)

32

29.5

8.8

1.5

29.4

24.4, 33.4

14.7, 55.1


Plasmacytoid DC (Live Scatter)

32

0.4

0.2

0.0

0.4

0.3, 0.5

0.1, 0.7

Tcell

Activated CD4+ T cells

32

1.6

0.7

0.1

1.4

1.1, 2.0

0.7, 3.7


Activated CD8+ T cells

32

4.6

3.6

0.6

3.3

2.2, 5.9

0.6, 16.6


Activated Central Memory CD4+ T cells

32

1.8

0.7

0.1

1.7

1.2, 2.3

0.6, 3.4


Activated Central Memory CD8+ T cells

32

8.6

5.0

0.9

7.0

4.9, 11.3

1.8, 21.4


Activated Effector CD4+ T cells

32

3.6

3.1

0.6

2.4

1.5, 5.1

0.3, 15.0


Activated Effector CD8+ T cells

32

7.0

7.0

1.2

4.6

2.8, 9.3

1.0, 35.5


Activated Effector Memory CD4+ T cells

32

5.8

2.1

0.4

5.5

4.2, 7.1

2.8, 11.2


Activated Effector Memory CD8+ T cells

32

14.7

10.3

1.8

13.4

8.0, 16.6

3.6, 54.8


Activated Naive CD4+ T cells

32

0.8

0.5

0.1

0.6

0.4, 1.0

0.2, 2.5


Activated Naive CD8+ T cells

32

2.1

1.6

0.3

1.3

0.7, 3.0

0.2, 5.4


Average CD4+ T cells

32

47.9

7.0

1.2

48.3

43.8, 51.5

32.6, 63.0


Average Lymphocytes

32

69.7

7.8

1.4

70.2

66.9, 73.9

46.7, 85.5


Average T cells

32

77.0

5.5

1.0

75.5

74.1, 82.1

66.7, 86.8


CD8+ T cells

32

22.1

6.5

1.1

20.2

18.2, 24.8

12.5, 40.7


Central Memory CD4+ T cells

32

37.9

8.9

1.6

37.8

31.3, 44.5

23.2, 59.0


Central Memory CD8+ T cells

32

5.8

2.7

0.5

5.2

4.1, 7.4

1.7, 11.7


Effector CD4+ T cells

32

1.9

3.2

0.6

1.1

0.6, 1.8

0.1, 18.1


Effector CD8+ T cells

32

26.7

12.4

2.2

23.7

16.6, 34.2

10.8, 57.2


Effector Memory CD4+ T cells

32

8.8

4.7

0.8

8.4

5.9, 9.5

2.3, 26.8


Effector Memory CD8+ T cells

32

7.9

4.3

0.8

7.3

4.3, 10.6

2.4, 18.3


Naive CD4+ T cells

32

51.1

11.4

2.0

51.3

45.3, 58.6

26.1, 72.2


Naive CD8+ T cells

32

59.5

12.4

2.2

62.3

49.4, 68.8

29.7, 78.1

Tfh

Activated T follicular helper

32

0.6

0.3

0.0

0.5

0.4, 0.8

0.1, 1.2


Medium Activated T follicular helper

32

19.7

4.7

0.8

20.9

15.9, 22.9

11.0, 27.8


T follicular helper

32

12.0

3.2

0.6

12.2

9.9, 14.1

5.6, 20.6


T follicular helper type 1

32

27.8

5.5

1.0

26.6

23.7, 31.2

16.9, 39.0


T follicular helper type 17

32

31.0

6.0

1.1

30.0

27.6, 35.1

18.3, 41.8


T follicular helper type 2

32

27.3

5.8

1.0

27.0

23.6, 31.6

17.8, 40.0


T follicular regulatory

32

4.0

1.5

0.3

3.8

2.9, 5.2

1.2, 8.1

Treg

Activated Memory Treg cells

32

42.2

7.0

1.2

41.9

36.8, 47.9

31.1, 54.9


Activated Naïve Treg cells

32

2.6

2.6

0.5

1.9

1.1, 3.4

0.4, 14.5


Effector Treg cells

32

52.4

12.7

2.2

49.9

44.9, 63.0

28.6, 78.8


HLA-DR- Memory Treg cells

32

43.6

7.4

1.3

42.5

39.4, 49.9

28.9, 56.7


HLA-DR- Naive Treg cells

32

11.5

4.5

0.8

11.2

8.2, 15.3

3.6, 19.9


Treg cells

32

7.3

1.7

0.3

7.2

6.3, 8.7

3.5, 10.5

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile