Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 3. RIs and associated CIs for overall study population



Panel

Cell Subset

95% RI

90% CIs for RIs

Bcell

B cells

4.73-22.54

(4.04, 5.29) (19.90, 24.80)


IgD+ Memory B cells

1.78-17.50

(1.49, 2.07) (16.20, 18.30)


IgD- Memory B cells

7.04-40.87

(5.22, 8.59) (36.60, 45.40)


IgD-CD27- B cells

2.61-24.25

(2.16, 3.41) (21.70, 26.00)


Naïve B cells

31.82-83.77

(26.50, 38.70) (80.90, 85.70)


Plasma cells

0.22-3.40

(0.15, 0.28) (2.85, 4.18)


Plasmablasts

0.05-1.42

(0.03, 0.07) (1.13, 1.65)


Transitional B cells

0.63-12.03

(0.41, 0.87) (9.78, 17.10)

Tcell

Activated CD4+ T cells

0.76-4.10

(0.64, 0.86) (3.26, 4.80)


Activated CD8+ T cells

1.05-14.21

(0.74, 1.63) (11.40, 16.60)


Activated Central Memory CD4+ T cells

0.63-3.24

(0.56, 0.75) (2.80, 3.65)


Activated Central Memory CD8+ T cells

1.62-16.82

(1.40, 1.75) (14.10, 18.90)


Activated Effector CD4+ T cells

0.45-10.78

(0.35, 0.59) (9.17, 14.80)


Activated Effector CD8+ T cells

1.34-25.22

(1.10, 1.81) (18.70, 35.50)


Activated Effector Memory CD4+ T cells

2.17-12.19

(1.60, 2.63) (10.70, 14.00)


Activated Effector Memory CD8+ T cells

3.50-31.81

(3.12, 4.21) (25.60, 33.70)


Activated Naive CD4+ T cells

0.13-3.09

(0.11, 0.16) (2.34, 5.06)


Activated Naive CD8+ T cells

0.33-5.74

(0.27, 0.42) (5.40, 6.40)


Average CD4+ T cells

36.82-68.30

(34.20, 39.75) (65.70, 72.75)


Average Lymphocytes

43.12-78.72

(37.05, 47.10) (76.45, 80.83)


Average T cells

66.00-88.11

(64.57, 68.17) (87.23, 88.63)


CD8+ T cells

10.14-37.60

(9.00, 11.40) (33.70, 40.70)


Central Memory CD4+ T cells

24.72-67.63

(22.30, 27.30) (63.30, 69.60)


Central Memory CD8+ T cells

2.36-26.81

(1.98, 2.76) (24.30, 30.90)


Effector CD4+ T cells

0.16-14.41

(0.12, 0.21) (10.70, 18.10)


Effector CD8+ T cells

8.90-61.02

(7.20, 11.00) (55.60, 68.00)


Effector Memory CD4+ T cells

3.13-24.83

(2.29, 4.24) (18.40, 28.40)


Effector Memory CD8+ T cells

2.60-21.65

(2.42, 3.07) (19.30, 24.20)


Naive CD4+ T cells

17.25-66.45

(11.80, 19.00) (61.60, 72.20)


Naive CD8+ T cells

19.91-78.56

(17.40, 22.20) (74.40, 81.70)

Treg

Activated Memory Treg cells

31.06-64.35

(29.00, 33.60) (60.40, 67.70)


Activated Naïve Treg cells

0.24-9.25

(0.12, 0.38) (6.64, 14.50)


Effector Treg cells

36.79-89.96

(34.30, 42.80) (86.90, 91.60)


HLA-DR- Memory Treg cells

26.37-59.02

(24.20, 29.50) (56.00, 62.30)


HLA-DR- Naive Treg cells

2.64-19.76

(2.14, 3.35) (17.50, 21.50)


Treg cells

3.89-10.71

(3.35, 4.40) (10.30, 11.60)

DCMNK

CD11c+CD123+ DC (DC)

0.28-32.49

(0.20, 0.38) (22.50, 40.40)


CD11c+CD123+ DC (Live Scatter)

0.00-0.59

(0.00, 0.00) (0.38, 0.78)


CD14+ Monocytes

59.24-90.22

(54.30, 63.70) (88.80, 92.60)


CD16+CD14- Monocytes

1.25-23.57

(0.50, 1.89) (19.40, 29.50)


CD16+CD56+ NK cells

45.89-90.14

(35.80, 52.20) (89.00, 91.10)


CD16+CD56- NK cells

2.39-32.09

(2.25, 2.59) (25.60, 38.60)


CD16-CD56+ NK cells

4.81-38.56

(4.48, 5.04) (27.40, 59.50)


Classical Monocytes

56.81-89.01

(51.70, 61.40) (87.50, 92.00)


Dendritic cells (Lin-)

0.74-3.92

(0.60, 0.87) (3.12, 4.35)


Dendritic cells (Live Scatter)

0.58-2.51

(0.42, 0.68) (2.23, 2.63)


Monocytes

3.47-18.81

(2.81, 4.40) (16.80, 19.90)


Myeloid DC (DC)

23.16-69.50

(19.70, 28.20) (64.60, 72.70)


Myeloid DC (Live Scatter)

0.17-1.43

(0.11, 0.26) (1.21, 1.66)


NK cells

2.49-19.36

(2.21, 2.91) (17.30, 21.90)


Plasmacytoid DC (DC)

10.51-46.56

(8.96, 13.00) (41.00, 55.00)


Plasmacytoid DC (Live Scatter)

0.10-0.76

(0.08, 0.12) (0.65, 0.98)

Tfh

Activated T follicular helper

0.13-1.02

(0.11, 0.16) (0.88, 1.21)


Medium Activated T follicular helper

9.89-26.06

(8.82, 11.00) (24.80, 27.40)


T follicular helper

6.93-20.89

(6.37, 7.76) (19.70, 24.10)


T follicular helper type 1

13.60-37.99

(11.60, 16.70) (36.30, 39.20)


T follicular helper type 17

21.65-49.15

(21.10, 22.90) (46.10, 55.30)


T follicular helper type 2

15.03-34.58

(14.00, 16.20) (32.60, 39.90)


T follicular regulatory

2.11-9.75

(1.97, 2.29) (8.21, 12.20)

Summary Statistics Summary Statistics Summary Statistics


RI: reference interval; CI: confidence interval