Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2a. Descriptive Summary for Overall Study Population



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

127

11.0

4.6

0.4

10.2

7.8, 13.5

1.8, 29.1


IgD+ Memory B cells

127

7.4

4.5

0.4

6.4

4.3, 9.8

1.3, 28.0


IgD- Memory B cells

127

20.5

9.1

0.8

19.3

13.4, 25.7

4.2, 50.9


IgD-CD27- B cells

127

10.1

5.7

0.5

8.8

5.9, 12.8

1.4, 28.9


Naïve B cells

127

62.0

13.2

1.2

64.9

53.9, 70.6

20.9, 86.6


Plasma cells

127

1.2

0.9

0.1

0.8

0.5, 1.6

0.1, 5.0


Plasmablasts

127

0.4

0.4

0.0

0.3

0.2, 0.6

0.0, 2.3


Transitional B cells

127

4.2

3.2

0.3

3.6

2.1, 5.6

0.4, 21.1

DCMNK

CD11c+CD123+ DC (DC)

127

6.8

9.2

0.8

3.0

1.0, 8.9

0.1, 52.4


CD11c+CD123+ DC (Live Scatter)

127

0.1

0.2

0.0

0.0

0.0, 0.1

0.0, 0.8


CD14+ Monocytes

127

79.6

8.1

0.7

80.8

75.9, 85.8

53.7, 93.1


CD16+CD14- Monocytes

127

8.9

6.0

0.5

7.5

4.4, 12.3

0.3, 30.2


CD16+CD56+ NK cells

127

75.8

12.1

1.1

79.1

68.8, 84.9

25.4, 91.1


CD16+CD56- NK cells

127

10.0

7.7

0.7

8.0

4.8, 12.5

1.8, 39.5


CD16-CD56+ NK cells

127

13.3

9.3

0.8

11.0

7.4, 16.5

3.7, 65.7


Classical Monocytes

127

77.3

8.6

0.8

78.0

73.4, 84.0

51.3, 92.3


Dendritic cells (Lin-)

127

1.9

0.8

0.1

1.7

1.3, 2.3

0.4, 4.7


Dendritic cells (Live Scatter)

127

1.4

0.5

0.0

1.3

1.0, 1.7

0.3, 2.7


Monocytes

127

10.5

4.1

0.4

10.2

7.5, 14.2

2.4, 23.2


Myeloid DC (DC)

127

46.5

11.9

1.1

46.7

37.4, 54.3

18.8, 75.7


Myeloid DC (Live Scatter)

127

0.6

0.3

0.0

0.6

0.4, 0.8

0.1, 1.8


NK cells

127

8.7

4.7

0.4

7.8

5.1, 11.4

2.1, 24.3


Plasmacytoid DC (DC)

127

25.4

10.1

0.9

23.9

17.5, 32.7

3.9, 55.1


Plasmacytoid DC (Live Scatter)

127

0.3

0.2

0.0

0.3

0.2, 0.4

0.1, 1.0

Tcell

Activated CD4+ T cells

123

1.8

0.9

0.1

1.6

1.2, 2.1

0.4, 5.8


Activated CD8+ T cells

123

4.9

3.5

0.3

3.8

2.5, 6.3

0.6, 19.0


Activated Central Memory CD4+ T cells

123

1.6

0.7

0.1

1.5

1.1, 2.0

0.4, 3.7


Activated Central Memory CD8+ T cells

122

5.9

3.9

0.4

4.6

3.1, 7.5

1.2, 21.4


Activated Effector CD4+ T cells

123

3.7

2.9

0.3

2.8

1.6, 5.5

0.3, 15.0


Activated Effector CD8+ T cells

122

7.5

6.5

0.6

5.1

3.5, 9.9

1.0, 38.4


Activated Effector Memory CD4+ T cells

123

5.8

2.8

0.3

5.3

3.8, 6.8

1.5, 21.8


Activated Effector Memory CD8+ T cells

122

12.2

7.9

0.7

10.5

6.8, 15.3

1.4, 54.8


Activated Naive CD4+ T cells

123

1.0

0.9

0.1

0.8

0.4, 1.4

0.1, 5.2


Activated Naive CD8+ T cells

122

2.0

1.6

0.1

1.4

0.8, 2.8

0.2, 7.9


Average CD4+ T cells

127

52.1

8.3

0.7

52.1

45.8, 57.8

32.6, 72.8


Average Lymphocytes

127

64.0

9.7

0.9

65.9

59.6, 70.9

34.5, 85.5


Average T cells

127

77.8

6.3

0.6

78.0

73.3, 82.7

64.1, 93.0


CD8+ T cells

123

21.1

6.9

0.6

20.7

16.5, 24.2

5.9, 45.4


Central Memory CD4+ T cells

123

45.6

11.4

1.0

46.6

37.7, 53.3

21.8, 77.6


Central Memory CD8+ T cells

122

10.7

6.5

0.6

9.4

5.7, 14.0

1.7, 32.8


Effector CD4+ T cells

123

2.8

4.1

0.4

1.2

0.6, 3.2

0.0, 25.5


Effector CD8+ T cells

122

29.5

14.9

1.3

26.1

17.4, 39.9

1.7, 73.0


Effector Memory CD4+ T cells

123

10.9

6.0

0.5

9.8

6.6, 13.4

2.3, 47.3


Effector Memory CD8+ T cells

122

10.4

5.7

0.5

9.8

5.9, 15.1

2.3, 33.6


Naive CD4+ T cells

123

40.4

14.4

1.3

39.5

27.1, 51.9

6.0, 73.6


Naive CD8+ T cells

122

49.4

17.3

1.6

49.8

34.3, 64.3

16.4, 83.0

Tfh

Activated T follicular helper

127

0.5

0.2

0.0

0.4

0.3, 0.6

0.1, 1.3


Medium Activated T follicular helper

127

17.6

4.4

0.4

16.8

14.4, 20.9

8.1, 27.8


T follicular helper

127

12.9

3.8

0.3

12.8

10.0, 14.6

5.6, 24.3


T follicular helper type 1

127

25.9

6.2

0.6

25.8

22.1, 29.5

7.4, 42.1


T follicular helper type 17

127

33.8

7.2

0.6

33.8

28.4, 38.3

18.3, 59.1


T follicular helper type 2

127

24.5

5.5

0.5

23.9

20.8, 28.4

10.6, 40.0


T follicular regulatory

127

4.7

2.1

0.2

4.4

3.1, 5.6

1.2, 13.6

Treg

Activated Memory Treg cells

127

46.1

8.6

0.8

45.9

40.1, 51.3

24.7, 70.1


Activated Naïve Treg cells

127

2.3

2.6

0.2

1.4

0.7, 2.9

0.1, 17.0


Effector Treg cells

127

64.6

13.7

1.2

65.0

56.9, 74.7

28.6, 92.1


HLA-DR- Memory Treg cells

127

42.8

8.6

0.8

42.2

37.6, 48.8

21.1, 67.0


HLA-DR- Naive Treg cells

127

8.9

4.7

0.4

7.8

5.3, 11.3

1.2, 32.8


Treg cells

127

7.3

1.8

0.2

7.1

6.2, 8.4

3.2, 14.4

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile