Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2o. Descriptive Summary for Male Subjects Aged 50-56 Years



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

17

10.4

2.8

0.7

10.5

7.8, 12.0

6.9, 15.7


IgD+ Memory B cells

17

8.4

4.8

1.2

8.0

4.0, 12.2

1.7, 17.8


IgD- Memory B cells

17

18.7

10.1

2.4

15.6

12.1, 22.6

4.2, 45.4


IgD-CD27- B cells

17

8.7

5.8

1.4

6.7

4.8, 12.5

2.2, 22.8


Naïve B cells

17

64.2

14.7

3.6

66.4

61.4, 70.6

26.5, 85.7


Plasma cells

17

0.7

0.4

0.1

0.7

0.5, 0.9

0.2, 1.6


Plasmablasts

17

0.3

0.2

0.0

0.2

0.2, 0.4

0.1, 0.8


Transitional B cells

17

3.3

1.9

0.5

3.1

2.2, 3.9

0.8, 7.9

DCMNK

CD11c+CD123+ DC (DC)

17

6.7

12.5

3.0

2.4

1.0, 6.8

0.3, 52.4


CD11c+CD123+ DC (Live Scatter)

17

0.1

0.2

0.0

0.0

0.0, 0.1

0.0, 0.8


CD14+ Monocytes

17

79.9

8.0

1.9

81.0

73.4, 86.5

66.5, 92.6


CD16+CD14- Monocytes

17

8.7

4.8

1.2

8.0

6.6, 9.6

1.9, 21.7


CD16+CD56+ NK cells

17

77.1

13.0

3.1

80.8

66.6, 88.1

45.0, 90.3


CD16+CD56- NK cells

17

11.2

8.7

2.1

8.2

6.0, 14.7

1.8, 30.9


CD16-CD56+ NK cells

17

10.7

5.3

1.3

8.8

7.2, 12.1

4.7, 22.5


Classical Monocytes

17

77.5

8.7

2.1

79.4

72.5, 83.7

60.4, 92.0


Dendritic cells (Lin-)

17

2.0

0.9

0.2

1.8

1.3, 2.3

0.7, 4.4


Dendritic cells (Live Scatter)

17

1.4

0.6

0.1

1.4

1.0, 1.6

0.6, 2.6


Monocytes

17

11.1

5.4

1.3

11.5

6.1, 14.5

3.3, 23.2


Myeloid DC (DC)

17

45.6

12.6

3.1

44.6

37.4, 54.3

19.7, 64.6


Myeloid DC (Live Scatter)

17

0.6

0.3

0.1

0.6

0.5, 0.7

0.1, 1.4


NK cells

17

8.5

4.8

1.2

8.5

4.2, 11.2

2.2, 19.4


Plasmacytoid DC (DC)

17

26.7

10.7

2.6

26.2

20.3, 32.7

3.9, 44.1


Plasmacytoid DC (Live Scatter)

17

0.4

0.2

0.1

0.3

0.2, 0.5

0.1, 1.0

Tcell

Activated CD4+ T cells

16

2.0

1.1

0.3

1.6

1.4, 2.2

0.9, 4.6


Activated CD8+ T cells

16

6.1

4.1

1.0

4.9

2.8, 8.1

0.7, 15.8


Activated Central Memory CD4+ T cells

16

1.9

0.9

0.2

1.8

1.2, 2.5

0.7, 3.7


Activated Central Memory CD8+ T cells

16

4.9

3.0

0.7

3.8

2.9, 7.3

1.4, 11.2


Activated Effector CD4+ T cells

16

3.9

2.8

0.7

3.0

1.7, 6.3

0.9, 9.7


Activated Effector CD8+ T cells

16

8.3

5.1

1.3

5.8

4.7, 10.4

2.9, 21.4


Activated Effector Memory CD4+ T cells

16

7.3

3.6

0.9

6.5

4.5, 10.3

2.6, 14.0


Activated Effector Memory CD8+ T cells

16

12.6

7.1

1.8

10.2

7.7, 15.1

5.4, 33.7


Activated Naive CD4+ T cells

16

1.3

1.3

0.3

0.9

0.3, 1.7

0.2, 5.1


Activated Naive CD8+ T cells

16

2.8

2.1

0.5

2.6

0.8, 4.1

0.3, 7.9


Average CD4+ T cells

17

57.9

9.0

2.2

60.1

54.7, 60.7

35.8, 72.8


Average Lymphocytes

17

56.4

12.1

2.9

53.8

47.7, 67.4

34.5, 73.0


Average T cells

17

79.3

4.7

1.1

78.5

75.9, 84.1

70.3, 85.8


CD8+ T cells

16

19.4

8.8

2.2

20.4

11.3, 24.6

5.9, 37.2


Central Memory CD4+ T cells

16

48.0

12.6

3.2

49.0

38.0, 57.0

26.7, 69.6


Central Memory CD8+ T cells

16

13.5

7.7

1.9

13.3

7.2, 17.1

3.9, 32.8


Effector CD4+ T cells

16

4.0

6.5

1.6

1.4

0.6, 5.5

0.0, 25.5


Effector CD8+ T cells

16

37.1

20.8

5.2

32.8

24.0, 52.4

1.7, 73.0


Effector Memory CD4+ T cells

16

8.9

5.0

1.2

7.7

5.4, 12.8

2.3, 18.3


Effector Memory CD8+ T cells

16

8.6

5.0

1.3

7.4

4.9, 10.7

2.5, 18.7


Naive CD4+ T cells

16

38.7

15.3

3.8

41.1

23.7, 50.2

17.9, 60.3


Naive CD8+ T cells

16

40.8

19.7

4.9

35.8

23.3, 51.8

16.4, 81.7

Tfh

Activated T follicular helper

17

0.4

0.2

0.0

0.4

0.3, 0.5

0.1, 0.8


Medium Activated T follicular helper

17

15.9

5.2

1.3

15.6

11.8, 19.7

8.1, 25.5


T follicular helper

17

12.9

4.4

1.1

11.5

9.4, 17.4

6.4, 20.2


T follicular helper type 1

17

23.6

7.8

1.9

24.3

21.1, 28.6

7.4, 37.7


T follicular helper type 17

17

36.0

9.6

2.3

33.4

29.9, 40.1

22.3, 59.1


T follicular helper type 2

17

24.2

6.3

1.5

24.6

22.3, 29.3

10.6, 33.0


T follicular regulatory

17

5.0

2.9

0.7

4.1

3.1, 5.4

2.2, 13.6

Treg

Activated Memory Treg cells

17

49.7

10.4

2.5

49.3

44.4, 54.7

29.0, 70.1


Activated Naïve Treg cells

17

3.3

4.0

1.0

2.0

1.3, 2.8

1.0, 17.0


Effector Treg cells

17

70.8

13.0

3.1

68.2

64.4, 80.1

42.8, 91.0


HLA-DR- Memory Treg cells

17

36.9

8.8

2.1

35.5

30.6, 44.1

21.1, 51.0


HLA-DR- Naive Treg cells

17

10.0

6.5

1.6

9.8

6.2, 10.8

4.4, 32.8


Treg cells

17

7.7

1.7

0.4

7.5

6.5, 8.8

5.3, 10.5

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile