Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2l. Descriptive Summary for Male Subjects Aged 18-29 Years



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

16

10.4

3.3

0.8

9.5

8.1, 13.4

5.3, 16.4


IgD+ Memory B cells

16

7.0

3.8

0.9

6.0

4.3, 9.7

2.1, 16.2


IgD- Memory B cells

16

26.7

11.2

2.8

26.9

22.4, 33.1

8.3, 50.9


IgD-CD27- B cells

16

16.6

7.3

1.8

16.4

12.2, 22.7

3.9, 28.9


Naïve B cells

16

49.8

14.6

3.6

47.0

40.6, 59.1

20.9, 75.1


Plasma cells

16

2.0

0.8

0.2

1.7

1.4, 2.7

0.5, 3.7


Plasmablasts

16

0.6

0.3

0.1

0.6

0.4, 0.9

0.0, 1.0


Transitional B cells

16

2.9

2.5

0.6

2.7

1.1, 4.2

0.4, 10.5

DCMNK

CD11c+CD123+ DC (DC)

16

2.9

2.3

0.6

2.9

1.2, 3.2

0.6, 9.7


CD11c+CD123+ DC (Live Scatter)

16

0.0

0.0

0.0

0.0

0.0, 0.1

0.0, 0.2


CD14+ Monocytes

16

78.7

8.9

2.2

79.0

75.4, 85.9

53.7, 88.2


CD16+CD14- Monocytes

16

9.0

7.4

1.9

7.5

4.6, 11.8

1.8, 30.2


CD16+CD56+ NK cells

16

79.4

7.6

1.9

82.2

73.9, 85.3

63.0, 87.8


CD16+CD56- NK cells

16

6.3

4.3

1.1

5.5

3.0, 7.8

2.3, 17.2


CD16-CD56+ NK cells

16

13.6

7.4

1.8

11.3

8.4, 18.0

4.5, 33.7


Classical Monocytes

16

76.7

9.5

2.4

77.4

72.8, 85.0

51.7, 87.1


Dendritic cells (Lin-)

16

2.0

0.8

0.2

1.7

1.5, 2.4

0.8, 4.2


Dendritic cells (Live Scatter)

16

1.5

0.5

0.1

1.4

1.1, 1.8

0.7, 2.5


Monocytes

16

8.7

3.4

0.8

9.1

5.2, 11.0

3.3, 14.4


Myeloid DC (DC)

16

45.3

12.4

3.1

42.9

37.6, 54.5

22.6, 70.5


Myeloid DC (Live Scatter)

16

0.7

0.4

0.1

0.7

0.4, 0.8

0.2, 1.8


NK cells

16

8.3

4.5

1.1

7.8

5.0, 11.2

2.9, 19.8


Plasmacytoid DC (DC)

16

29.3

9.8

2.4

29.2

23.3, 32.8

14.7, 55.1


Plasmacytoid DC (Live Scatter)

16

0.4

0.2

0.0

0.4

0.3, 0.6

0.1, 0.7

Tcell

Activated CD4+ T cells

16

1.5

0.7

0.2

1.4

1.0, 1.8

0.7, 3.7


Activated CD8+ T cells

16

3.9

3.5

0.9

2.6

2.0, 4.0

0.6, 12.3


Activated Central Memory CD4+ T cells

16

1.7

0.7

0.2

1.7

1.2, 2.1

0.6, 3.4


Activated Central Memory CD8+ T cells

16

7.6

4.8

1.2

5.4

4.7, 11.3

1.8, 17.8


Activated Effector CD4+ T cells

16

3.8

3.9

1.0

2.1

1.3, 4.9

0.6, 15.0


Activated Effector CD8+ T cells

16

5.1

5.1

1.3

3.7

2.2, 5.3

1.0, 19.3


Activated Effector Memory CD4+ T cells

16

5.4

1.4

0.3

5.5

4.2, 6.5

3.3, 7.8


Activated Effector Memory CD8+ T cells

16

11.4

6.5

1.6

11.9

5.9, 15.7

3.6, 27.6


Activated Naive CD4+ T cells

16

0.7

0.4

0.1

0.6

0.4, 1.0

0.2, 1.5


Activated Naive CD8+ T cells

16

1.9

1.7

0.4

1.2

0.7, 2.6

0.2, 5.4


Average CD4+ T cells

16

48.5

8.0

2.0

48.2

43.2, 53.6

34.2, 63.0


Average Lymphocytes

16

69.9

8.8

2.2

71.2

66.9, 73.7

46.7, 85.5


Average T cells

16

77.4

5.0

1.2

76.0

74.3, 80.2

68.2, 86.8


CD8+ T cells

16

21.8

7.3

1.8

19.6

17.7, 23.3

13.8, 40.7


Central Memory CD4+ T cells

16

38.6

8.6

2.2

37.0

31.8, 45.1

25.1, 54.7


Central Memory CD8+ T cells

16

5.5

2.5

0.6

5.1

4.2, 6.1

1.7, 11.7


Effector CD4+ T cells

16

2.3

4.3

1.1

1.4

0.4, 2.0

0.1, 18.1


Effector CD8+ T cells

16

27.6

13.7

3.4

23.7

16.4, 37.8

10.8, 57.2


Effector Memory CD4+ T cells

16

9.1

6.1

1.5

7.5

5.3, 9.6

2.3, 26.8


Effector Memory CD8+ T cells

16

7.7

4.4

1.1

5.4

4.3, 10.7

3.4, 16.4


Naive CD4+ T cells

16

49.7

13.0

3.2

51.8

43.5, 57.4

26.1, 72.2


Naive CD8+ T cells

16

58.9

14.9

3.7

64.5

46.4, 70.7

29.7, 77.2

Tfh

Activated T follicular helper

16

0.6

0.3

0.1

0.6

0.4, 0.8

0.1, 1.0


Medium Activated T follicular helper

16

19.1

5.2

1.3

21.4

14.3, 22.7

11.0, 26.6


T follicular helper

16

12.5

3.5

0.9

12.6

10.9, 14.5

5.6, 20.6


T follicular helper type 1

16

25.8

3.6

0.9

26.0

22.8, 27.3

20.8, 35.3


T follicular helper type 17

16

32.4

4.9

1.2

31.4

28.7, 35.6

24.2, 41.8


T follicular helper type 2

16

27.9

5.9

1.5

27.5

23.3, 32.0

19.5, 39.9


T follicular regulatory

16

4.3

1.7

0.4

4.0

2.9, 5.6

2.0, 8.1

Treg

Activated Memory Treg cells

16

40.4

6.4

1.6

40.7

34.2, 44.3

32.2, 54.9


Activated Naïve Treg cells

16

3.3

3.3

0.8

2.5

1.2, 4.1

0.7, 14.5


Effector Treg cells

16

51.3

13.0

3.2

47.4

41.6, 64.1

28.6, 68.5


HLA-DR- Memory Treg cells

16

43.4

7.1

1.8

42.2

38.8, 47.8

28.9, 56.7


HLA-DR- Naive Treg cells

16

12.8

5.0

1.2

12.7

9.3, 17.3

3.6, 19.9


Treg cells

16

8.1

1.7

0.4

8.4

7.2, 9.1

4.4, 10.5

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile