Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2t. Descriptive Summary for Male, Caucasian Subjects



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

24

9.4

4.0

0.8

8.0

7.2, 10.4

4.6, 21.9


IgD+ Memory B cells

24

8.1

4.4

0.9

6.7

4.4, 9.9

2.2, 17.8


IgD- Memory B cells

24

17.4

8.4

1.7

15.0

11.7, 24.3

4.2, 34.4


IgD-CD27- B cells

24

8.3

4.5

0.9

7.3

5.1, 12.0

1.4, 17.3


Naïve B cells

24

66.2

10.9

2.2

65.4

56.2, 74.3

48.1, 85.7


Plasma cells

24

1.0

0.9

0.2

0.7

0.4, 1.6

0.1, 3.7


Plasmablasts

24

0.4

0.4

0.1

0.2

0.1, 0.8

0.0, 1.2


Transitional B cells

24

3.7

2.1

0.4

3.1

2.0, 4.9

0.8, 7.9

DCMNK

CD11c+CD123+ DC (DC)

24

2.9

3.7

0.8

1.4

0.9, 3.6

0.2, 16.8


CD11c+CD123+ DC (Live Scatter)

24

0.0

0.1

0.0

0.0

0.0, 0.1

0.0, 0.3


CD14+ Monocytes

24

85.3

4.2

0.9

86.2

84.4, 87.8

71.7, 92.6


CD16+CD14- Monocytes

24

4.1

2.6

0.5

3.6

2.1, 5.3

0.3, 9.6


CD16+CD56+ NK cells

24

78.0

13.1

2.7

82.2

74.4, 85.2

35.8, 90.3


CD16+CD56- NK cells

24

5.6

3.0

0.6

5.3

2.9, 7.6

1.8, 12.7


CD16-CD56+ NK cells

24

15.4

12.3

2.5

11.3

9.2, 17.9

5.5, 59.5


Classical Monocytes

24

83.8

4.4

0.9

84.3

82.8, 86.4

69.7, 92.0


Dendritic cells (Lin-)

24

2.1

0.9

0.2

2.0

1.5, 2.7

0.7, 4.4


Dendritic cells (Live Scatter)

24

1.5

0.5

0.1

1.4

1.1, 1.8

0.6, 2.6


Monocytes

24

11.8

3.5

0.7

12.0

8.8, 14.5

4.1, 18.7


Myeloid DC (DC)

24

50.4

12.3

2.5

53.3

44.4, 57.9

19.7, 75.7


Myeloid DC (Live Scatter)

24

0.8

0.3

0.1

0.8

0.6, 0.9

0.1, 1.7


NK cells

24

8.0

4.1

0.8

7.3

4.9, 9.8

2.1, 19.4


Plasmacytoid DC (DC)

24

26.5

9.6

2.0

27.7

19.3, 34.0

9.0, 44.1


Plasmacytoid DC (Live Scatter)

24

0.4

0.2

0.0

0.3

0.3, 0.5

0.1, 1.0

Tcell

Activated CD4+ T cells

23

1.5

0.6

0.1

1.4

0.9, 1.8

0.8, 3.5


Activated CD8+ T cells

23

4.4

3.9

0.8

2.9

2.0, 6.1

0.7, 15.8


Activated Central Memory CD4+ T cells

23

1.6

0.7

0.1

1.5

1.1, 2.0

0.6, 3.7


Activated Central Memory CD8+ T cells

23

5.6

4.4

0.9

4.3

2.5, 8.4

1.4, 17.8


Activated Effector CD4+ T cells

23

4.4

3.6

0.7

3.6

1.7, 6.7

0.9, 15.0


Activated Effector CD8+ T cells

23

6.5

5.7

1.2

4.3

2.5, 9.0

1.2, 21.4


Activated Effector Memory CD4+ T cells

23

6.0

3.0

0.6

5.0

4.1, 6.9

2.1, 14.0


Activated Effector Memory CD8+ T cells

23

10.5

7.7

1.6

7.6

5.2, 12.8

4.1, 33.7


Activated Naive CD4+ T cells

23

0.7

0.5

0.1

0.5

0.3, 0.9

0.1, 2.1


Activated Naive CD8+ T cells

23

1.8

1.7

0.3

1.1

0.6, 3.3

0.3, 5.6


Average CD4+ T cells

24

54.4

8.3

1.7

55.2

49.8, 60.2

39.8, 72.8


Average Lymphocytes

24

62.9

9.8

2.0

63.4

55.6, 70.4

44.4, 80.8


Average T cells

24

79.2

5.4

1.1

80.4

75.4, 83.8

68.3, 87.2


CD8+ T cells

23

20.3

8.9

1.9

18.3

15.5, 24.2

5.9, 45.4


Central Memory CD4+ T cells

23

43.1

11.9

2.5

45.0

32.9, 50.8

21.8, 66.2


Central Memory CD8+ T cells

23

13.4

7.8

1.6

11.7

6.4, 18.8

2.6, 26.7


Effector CD4+ T cells

23

2.5

4.5

0.9

0.7

0.3, 2.1

0.0, 17.3


Effector CD8+ T cells

23

24.6

15.2

3.2

22.8

12.9, 28.6

1.7, 68.0


Effector Memory CD4+ T cells

23

8.6

4.6

1.0

8.5

5.0, 11.7

2.3, 16.3


Effector Memory CD8+ T cells

23

10.9

5.3

1.1

10.1

6.7, 15.5

3.1, 20.7


Naive CD4+ T cells

23

45.4

15.9

3.3

45.9

33.2, 59.0

18.3, 73.6


Naive CD8+ T cells

23

51.1

19.2

4.0

49.8

33.9, 70.7

16.4, 81.7

Tfh

Activated T follicular helper

24

0.4

0.3

0.1

0.4

0.2, 0.5

0.1, 1.3


Medium Activated T follicular helper

24

17.4

4.1

0.8

17.6

14.8, 20.7

9.8, 25.6


T follicular helper

24

11.3

3.6

0.7

10.9

8.5, 14.3

5.6, 18.7


T follicular helper type 1

24

28.3

4.5

0.9

28.7

25.5, 30.7

19.6, 37.7


T follicular helper type 17

24

30.2

4.5

0.9

30.6

27.9, 33.3

21.1, 38.2


T follicular helper type 2

24

26.4

4.5

0.9

24.8

22.7, 31.8

18.6, 33.3


T follicular regulatory

24

5.7

2.5

0.5

4.8

4.2, 7.4

2.9, 13.6

Treg

Activated Memory Treg cells

24

47.6

9.6

2.0

44.9

42.3, 54.2

32.2, 70.1


Activated Naïve Treg cells

24

2.2

2.5

0.5

1.4

0.8, 2.0

0.4, 9.0


Effector Treg cells

24

61.9

15.7

3.2

64.2

49.8, 74.8

28.6, 90.4


HLA-DR- Memory Treg cells

24

41.7

9.0

1.8

41.9

34.9, 51.0

24.2, 56.7


HLA-DR- Naive Treg cells

24

8.4

3.6

0.7

7.9

4.9, 11.8

4.2, 15.3


Treg cells

24

7.4

1.7

0.3

7.1

6.4, 8.5

4.4, 11.6

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile