Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2i. Descriptive Summary for Female Subjects Aged 40-49 Years



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

14

10.4

5.3

1.4

10.0

6.2, 13.9

1.8, 23.7


IgD+ Memory B cells

14

6.4

4.7

1.3

5.1

2.4, 8.3

1.5, 17.5


IgD- Memory B cells

14

18.9

6.7

1.8

20.2

14.4, 23.6

7.3, 31.4


IgD-CD27- B cells

14

6.9

3.1

0.8

6.0

4.3, 10.4

3.6, 12.0


Naïve B cells

14

67.9

10.3

2.8

67.1

58.8, 74.9

53.6, 86.6


Plasma cells

14

1.0

0.9

0.2

0.6

0.5, 0.9

0.4, 2.9


Plasmablasts

14

0.4

0.3

0.1

0.2

0.2, 0.4

0.1, 1.3


Transitional B cells

14

7.3

5.7

1.5

5.0

3.5, 10.4

1.7, 21.1

DCMNK

CD11c+CD123+ DC (DC)

14

9.1

8.5

2.3

6.8

2.0, 16.1

0.3, 23.2


CD11c+CD123+ DC (Live Scatter)

14

0.1

0.1

0.0

0.1

0.0, 0.1

0.0, 0.4


CD14+ Monocytes

14

79.9

8.8

2.3

82.0

79.4, 84.8

54.3, 90.4


CD16+CD14- Monocytes

14

11.5

6.4

1.7

10.1

5.2, 18.1

3.8, 22.5


CD16+CD56+ NK cells

14

79.9

9.5

2.5

81.5

74.9, 88.2

55.3, 91.1


CD16+CD56- NK cells

14

9.6

7.2

1.9

7.7

5.0, 10.5

3.6, 29.1


CD16-CD56+ NK cells

14

9.7

5.0

1.3

7.5

5.0, 14.4

3.7, 17.3


Classical Monocytes

14

76.0

9.7

2.6

78.5

70.3, 82.0

51.3, 88.0


Dendritic cells (Lin-)

14

1.5

0.6

0.2

1.3

1.2, 2.3

0.9, 2.5


Dendritic cells (Live Scatter)

14

1.1

0.4

0.1

0.9

0.8, 1.6

0.7, 1.8


Monocytes

14

11.3

3.5

0.9

11.2

8.7, 14.2

5.4, 18.4


Myeloid DC (DC)

14

39.7

10.3

2.8

37.9

33.7, 49.2

18.8, 55.0


Myeloid DC (Live Scatter)

14

0.4

0.2

0.1

0.3

0.3, 0.6

0.2, 0.9


NK cells

14

10.0

5.7

1.5

10.8

4.0, 14.2

2.5, 21.9


Plasmacytoid DC (DC)

14

27.8

12.9

3.5

26.0

14.5, 37.8

12.3, 55.0


Plasmacytoid DC (Live Scatter)

14

0.3

0.1

0.0

0.3

0.2, 0.3

0.1, 0.6

Tcell

Activated CD4+ T cells

12

1.8

1.4

0.4

1.4

1.1, 1.8

0.6, 5.8


Activated CD8+ T cells

12

5.5

3.9

1.1

3.9

2.3, 9.5

1.9, 13.3


Activated Central Memory CD4+ T cells

12

1.6

0.9

0.3

1.4

0.8, 2.2

0.7, 3.7


Activated Central Memory CD8+ T cells

11

5.5

2.7

0.8

5.1

3.2, 8.1

1.9, 9.4


Activated Effector CD4+ T cells

12

3.8

2.3

0.7

3.9

2.0, 4.9

1.1, 9.2


Activated Effector CD8+ T cells

11

9.2

6.0

1.8

7.9

3.7, 14.8

2.3, 18.7


Activated Effector Memory CD4+ T cells

12

6.6

5.2

1.5

5.3

4.0, 6.1

2.2, 21.8


Activated Effector Memory CD8+ T cells

11

15.3

9.5

2.9

11.4

7.5, 22.8

3.4, 33.7


Activated Naive CD4+ T cells

12

0.7

0.8

0.2

0.4

0.1, 1.0

0.1, 3.1


Activated Naive CD8+ T cells

11

1.6

1.5

0.4

1.3

0.5, 2.2

0.3, 5.5


Average CD4+ T cells

14

55.4

8.6

2.3

58.4

47.0, 63.2

40.8, 66.9


Average Lymphocytes

14

65.1

4.3

1.1

64.5

61.7, 67.4

60.8, 76.7


Average T cells

14

78.4

7.8

2.1

76.4

72.5, 85.8

66.6, 93.0


CD8+ T cells

12

19.4

3.3

0.9

20.4

18.0, 21.2

11.5, 23.4


Central Memory CD4+ T cells

12

42.7

9.4

2.7

43.9

37.4, 47.4

22.3, 55.7


Central Memory CD8+ T cells

11

11.9

6.2

1.9

12.7

6.2, 15.9

3.5, 24.1


Effector CD4+ T cells

12

3.1

3.3

1.0

2.3

0.6, 3.7

0.2, 10.3


Effector CD8+ T cells

11

26.5

15.0

4.5

20.6

16.3, 34.4

12.2, 61.3


Effector Memory CD4+ T cells

12

11.0

7.0

2.0

9.8

6.1, 13.7

2.9, 28.4


Effector Memory CD8+ T cells

11

13.0

7.3

2.2

10.3

8.9, 19.9

2.6, 24.2


Naive CD4+ T cells

12

42.9

10.9

3.2

42.1

33.9, 53.9

26.8, 59.7


Naive CD8+ T cells

11

48.6

14.5

4.4

50.1

47.0, 53.0

22.2, 78.6

Tfh

Activated T follicular helper

14

0.4

0.2

0.0

0.4

0.3, 0.5

0.2, 0.7


Medium Activated T follicular helper

14

16.1

3.6

0.9

15.9

14.0, 18.0

8.8, 22.0


T follicular helper

14

12.3

3.3

0.9

12.2

9.5, 14.3

7.9, 18.3


T follicular helper type 1

14

26.3

5.1

1.4

25.8

23.5, 28.9

16.7, 36.3


T follicular helper type 17

14

34.4

6.6

1.8

35.0

33.3, 38.7

21.2, 45.9


T follicular helper type 2

14

22.2

5.3

1.4

21.0

17.9, 26.2

14.8, 31.6


T follicular regulatory

14

5.8

1.8

0.5

5.6

5.0, 6.9

3.0, 10.1

Treg

Activated Memory Treg cells

14

44.7

9.5

2.5

43.0

38.8, 50.5

24.7, 63.4


Activated Naïve Treg cells

14

1.2

1.9

0.5

0.5

0.4, 0.8

0.1, 7.6


Effector Treg cells

14

67.8

11.8

3.2

69.0

60.0, 76.2

45.0, 86.9


HLA-DR- Memory Treg cells

14

47.2

10.0

2.7

48.3

40.4, 55.6

31.6, 67.0


HLA-DR- Naive Treg cells

14

6.9

4.3

1.2

6.6

4.6, 7.8

2.7, 20.5


Treg cells

14

7.3

1.0

0.3

7.5

6.5, 8.2

5.2, 8.7

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile