Study to Define Adult Normative Ranges for Polychromatic Flow Cytometry Assays (Grifols)


Table 2p. Descriptive Summary for Caucasian Subjects



Panel

Cell Subset

N

Mean

SD

SE

Median

Q1, Q3

Min, Max

Bcell

B cells

51

9.3

3.7

0.5

8.4

6.9, 11.1

1.8, 21.9


IgD+ Memory B cells

51

9.1

4.4

0.6

8.1

5.9, 12.2

2.2, 18.3


IgD- Memory B cells

51

18.8

9.1

1.3

16.0

11.7, 24.5

4.2, 43.4


IgD-CD27- B cells

51

8.2

4.5

0.6

7.1

4.7, 12.0

1.4, 17.5


Naïve B cells

51

63.9

12.5

1.8

64.9

55.2, 73.6

26.8, 85.7


Plasma cells

51

0.9

0.7

0.1

0.7

0.4, 1.2

0.1, 3.7


Plasmablasts

51

0.4

0.3

0.0

0.2

0.2, 0.5

0.0, 1.3


Transitional B cells

51

4.1

2.6

0.4

3.8

2.1, 5.8

0.4, 11.7

DCMNK

CD11c+CD123+ DC (DC)

51

4.3

5.4

0.8

1.7

0.9, 5.7

0.1, 22.1


CD11c+CD123+ DC (Live Scatter)

51

0.1

0.1

0.0

0.0

0.0, 0.1

0.0, 0.4


CD14+ Monocytes

51

83.9

4.9

0.7

84.7

79.6, 87.5

71.7, 93.1


CD16+CD14- Monocytes

51

5.4

3.4

0.5

4.5

2.7, 7.2

0.3, 15.3


CD16+CD56+ NK cells

51

77.4

11.4

1.6

80.8

69.5, 85.5

35.8, 90.3


CD16+CD56- NK cells

51

7.3

5.7

0.8

5.9

3.8, 8.4

1.8, 31.9


CD16-CD56+ NK cells

51

14.5

9.8

1.4

11.6

7.4, 18.1

4.8, 59.5


Classical Monocytes

51

82.2

5.3

0.7

83.4

77.6, 86.0

69.7, 92.3


Dendritic cells (Lin-)

51

1.8

0.8

0.1

1.6

1.2, 2.3

0.6, 4.4


Dendritic cells (Live Scatter)

51

1.3

0.5

0.1

1.3

0.9, 1.6

0.4, 2.6


Monocytes

51

11.3

3.5

0.5

11.4

8.2, 14.4

4.1, 19.1


Myeloid DC (DC)

51

48.7

11.9

1.7

48.8

41.7, 56.5

19.7, 75.7


Myeloid DC (Live Scatter)

51

0.6

0.3

0.0

0.6

0.4, 0.8

0.1, 1.7


NK cells

51

8.3

4.8

0.7

7.1

4.8, 10.2

2.1, 24.3


Plasmacytoid DC (DC)

51

27.1

10.1

1.4

26.2

19.2, 36.0

9.0, 51.4


Plasmacytoid DC (Live Scatter)

51

0.3

0.2

0.0

0.3

0.2, 0.4

0.1, 1.0

Tcell

Activated CD4+ T cells

50

1.6

0.6

0.1

1.4

1.2, 1.8

0.6, 3.5


Activated CD8+ T cells

50

5.3

3.9

0.6

4.1

2.5, 8.0

0.7, 16.6


Activated Central Memory CD4+ T cells

50

1.7

0.7

0.1

1.5

1.1, 2.1

0.6, 3.7


Activated Central Memory CD8+ T cells

49

6.2

4.7

0.7

4.7

3.0, 8.2

1.4, 21.4


Activated Effector CD4+ T cells

50

4.2

3.1

0.4

3.6

1.7, 6.1

0.5, 15.0


Activated Effector CD8+ T cells

49

9.0

8.1

1.2

6.2

3.7, 11.8

1.2, 38.4


Activated Effector Memory CD4+ T cells

50

5.9

2.7

0.4

5.6

3.8, 7.1

1.6, 14.0


Activated Effector Memory CD8+ T cells

49

12.8

10.3

1.5

9.3

6.1, 17.8

3.1, 54.8


Activated Naive CD4+ T cells

50

0.8

0.6

0.1

0.6

0.4, 0.9

0.1, 2.3


Activated Naive CD8+ T cells

49

1.9

1.4

0.2

1.3

0.8, 2.9

0.3, 5.6


Average CD4+ T cells

51

54.2

7.7

1.1

54.4

49.4, 60.2

39.6, 72.8


Average Lymphocytes

51

63.9

8.9

1.2

65.2

59.2, 70.8

44.4, 80.8


Average T cells

51

79.4

6.4

0.9

80.3

74.6, 84.5

64.1, 93.0


CD8+ T cells

50

20.7

7.1

1.0

20.4

16.8, 23.3

5.9, 45.4


Central Memory CD4+ T cells

50

44.0

11.1

1.6

44.4

35.8, 51.9

21.8, 67.8


Central Memory CD8+ T cells

49

12.8

6.7

1.0

11.5

7.2, 16.6

2.6, 26.7


Effector CD4+ T cells

50

2.3

3.7

0.5

0.8

0.4, 2.2

0.0, 17.3


Effector CD8+ T cells

49

25.1

13.1

1.9

22.8

15.2, 34.2

1.7, 68.0


Effector Memory CD4+ T cells

50

9.2

5.0

0.7

8.5

5.4, 11.6

2.3, 28.4


Effector Memory CD8+ T cells

49

11.9

5.2

0.7

10.6

8.4, 15.5

3.1, 24.2


Naive CD4+ T cells

50

44.2

14.3

2.0

46.0

33.0, 56.0

18.3, 73.6


Naive CD8+ T cells

49

50.3

16.3

2.3

49.8

35.7, 63.1

16.4, 81.7

Tfh

Activated T follicular helper

51

0.4

0.3

0.0

0.4

0.3, 0.5

0.1, 1.3


Medium Activated T follicular helper

51

17.5

4.2

0.6

16.7

14.4, 20.7

8.8, 25.6


T follicular helper

51

11.4

3.2

0.5

11.2

8.5, 14.1

5.6, 18.7


T follicular helper type 1

51

30.4

5.2

0.7

29.5

26.9, 34.9

19.6, 42.1


T follicular helper type 17

51

29.2

5.1

0.7

29.5

24.4, 33.4

18.3, 38.7


T follicular helper type 2

51

25.4

5.0

0.7

24.2

21.6, 30.0

17.9, 40.0


T follicular regulatory

51

5.2

2.4

0.3

4.4

3.7, 5.9

2.2, 13.6

Treg

Activated Memory Treg cells

51

47.8

7.9

1.1

48.3

42.8, 52.3

31.1, 70.1


Activated Naïve Treg cells

51

1.7

1.9

0.3

1.3

0.7, 2.1

0.2, 9.0


Effector Treg cells

51

61.8

14.4

2.0

63.0

50.2, 74.7

28.6, 90.4


HLA-DR- Memory Treg cells

51

42.9

7.8

1.1

43.0

39.3, 48.0

24.2, 58.2


HLA-DR- Naive Treg cells

51

7.6

3.1

0.4

6.9

4.9, 9.1

2.8, 15.3


Treg cells

51

6.7

1.8

0.3

6.6

5.6, 8.1

3.2, 11.6

Summary Statistics


Two DCMNK gates are not reported because their values are ANR.


N: number of observations; SD: standard deviation; SE: standard error; Q1: 25th percentile; Q3: 75th percentile